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產(chǎn)品簡介
The DynaGen® Series bioreactors provide mechanical stimulation to three dimensional tissue engineered constructs to create physiological conditions in-vitro. The LigaGen system imparts axial stress or
詳細介紹
LigaGen Ligament & Tendon Bioreactor
The DynaGen Series
- 一,、美國tissuegrowth公司臨床相關(guān)的力學刺激組織工程生物反應器專家，世聯(lián)博研北京科技公司作為美國tissuegrowth在中國大陸**代理商,，簽約代理以下產(chǎn)品:
- 1,、三維力學加載細胞組織工程培養(yǎng)系統(tǒng)
- 2、三維軟骨力學刺激組織工程生物反應器
- 3,、3D血管組織工程搏動培養(yǎng)系統(tǒng)
- 4,、3D肌腱韌帶組織工程力學培養(yǎng)生物反應器
- 5、3D心臟瓣膜組織工程培養(yǎng)系統(tǒng)
- 6,、三維骨應力刺激組織工程生物反應器
- 7,、三維皮膚力學機械刺激組織工程生物反應器
- 8、3D灌注培養(yǎng)組織工程生物反應器
- 9、3d支架培養(yǎng)組織工程系統(tǒng)
- 10,、多樣品三維力學加載細胞組織組織工程培養(yǎng)系統(tǒng)
- 二,、世聯(lián)博研北京科技公司還簽約代理美國Flexcell公司細胞拉力、壓力及剪力仿真培養(yǎng)系統(tǒng)
- 是美國Flexcell在中國大陸**代理商,，簽約代理以下產(chǎn)品:
- 儀器部分:
- 1,、FX-5000C細胞壓力加載系統(tǒng)（flexcell FX5000 Compression system）
- 2、FX-5000T細胞應力加載系統(tǒng)（Flexcell FX5000 Tension system）
- 3,、Flex Jr. Tension顯微應力加載系統(tǒng)（Flex Jr. Tension）
- 4,、TissueTrain三維細胞組織培養(yǎng)系統(tǒng)（Flexcell Tissue® Train System）
- 5、STR-4000細胞流體切應力系統(tǒng)（Flexcell Fluid Shear Stress Device）
- 6,、應力/壓力傳導儀（FlexLink® FX-5000TFL/FX-5000CFL Tension or Compression）
- 7,、flexcell自動掃描設(shè)備和XyFlEX計算軟件（ScanFlex with XyFlex）
- 8、HiQFlowmate液體控系統(tǒng)（HIQ-4000-A/B/C HiQ Flowmate-Fluid Control System）
- 9,、OsciFlow_切應力模式控制器
- 10,、SFlex_SFlexJr_顯微應力加載系一號（StageFlexer StageFlexer Jr. Microscopy Tension Device）
- 11、FlexFlow顯微切應力加載設(shè)備二號（StageFlexer StageFlexer Microscopy Tension Device）
- 12,、STR-4000C 流體剪切系統(tǒng)W/O型電腦（Streamer Fluid Shear System w/o Computer）
- 13,、STR-4000D Streamer Fluid Shear Body Only
- 14、Flexcell 儲壓泵（Pressure Reservoir）
- 15,、FlexStop真空隔離閥
- flexcell培養(yǎng)耗材部分:
- 1,、TTPlate三維細胞培養(yǎng)板
- 2、BioFlex（flexcell BioFlex培養(yǎng)板）
- 5,、UniFlex（flexcell雙向應力培養(yǎng)板）
- 6,、BioPress壓力加載系統(tǒng)配套使用的BioPress培養(yǎng)板
- 7,、細胞培養(yǎng)載片
- 8,、圓形TTCircular三維細胞培養(yǎng)板
- 9、HI bioFlex培養(yǎng)板
- 10,、SFMembrane_硅膠膜
- Flexcell儀器,、配件銷售與租賃
三、世聯(lián)博研北京科技公司還簽約美國ebersmedical 一個簡單的機械性刺激細胞培養(yǎng)系統(tǒng),，簽約代理以下產(chǎn)品:
- 1,、TC-3 Bioreactor（簡單的機械性刺激細胞培養(yǎng)系統(tǒng)）
- A simple mechanical stimulation cell culture system at last!
- The TC-3 Bioreactor has been designed as a simple easy-to-use system suitable for cell culture
- under mechanical loading profiles defined by the user.
- It has been designed and manufactured thinking of the needs of
- researcher working on cell culture under mechanical stimuli.
- The TC-3 combines the features of a traditional testing machine with the particular demands of cell culture,
- with special emphasis on the parts sterilization, easy assembling,
- sample inspection by microscopy techniques and simple manipulation. Also scalability and
- versatility are key features of the TC-3, in which three grips models can be interchanged
- in order to adapt to the features of the testing substrate or scaffold.
- The system is controlled by a simple computer interface which allows defining the most
- common loading profiles which can be applied on the culture substrate.
- 2、TEB1000 flow bioreactor（TEB1000流體生物反應器）
- 3,、TC-3 mechanical stimulation bioreactor（TC-3機械性刺激生物反應器）
- 4,、P3D perfusion chambers（灌注細胞培養(yǎng)）
- 5、Vascular Chamber（血博灌注細胞培養(yǎng)）
四,、簽約代理美國B-Bridge細胞牽拉儀（STREX）
美國B-Bridge細胞牽拉儀（STREX）是一種模擬細胞生長過程中的機械應力裝置,。通過施加細胞應力，張力腔室中的種子細胞被拉伸或壓縮。施加在細胞上的機械應力更好的模擬了自然動態(tài)生理環(huán)境,。STREX可控制施加在細胞上的應力級別,，部分型號可與顯微鏡聯(lián)用。
ST-140-04
ST-140-10
See the links below for specific system configurations:
Vascular
HeartValve
Ligament
Cartilage
Bone
Skin
Custom
Watch a video of the LigaGen （L30-1） bioreactor.
The DynaGen® Series bioreactors provide mechanical stimulation to three dimensional tissue engineered constructs to create physiological conditions in-vitro. The LigaGen system imparts axial stress or strain to a ligament or tendon construct. The stress/strain profiles can be defined by the user and can be a simple harmonic （sinusoidal） or a physiological waveform. Applications include the investigation of cell function and differentiation, pharmaceutical benchtop testing, and the seeding and growth of engineered tissues and medical devices. Researchers are currently utilizing these systems in a wide range of research areas including:
- ACL regenerative medicine
- Collagen gel stimulation
- Small diameter tendon stimulation
- Hand tendon and ligament regenerative medicine
Chambers
The LigaGen bioreactor chamber delivers oscillatory axial stimulation to samples with large aspect ratios. The chambers can be used with a variety of construct materials from decellularized tendons and ligaments to polymerized hydrogels. The chamber accommodates samples up to 30mm in length and 3mm thickness. A wide range of construct geometries can be stimulated using multiple grip styles. A flexible bellows seal facilitates the transfer of axial motion into the chamber while maintaining a sterile environment. Single sample and multisample chamber configurations are provided and they can be used with a perfusion system to provide convective media transport around the samples. Fabricated from bioinert materials, the autoclavable chambers also polycarbonate removable windows for easy viewing and laser micrometer measurement. Additional chamber features include:
| L30-1X: | Single sample chamber with 23ml internal volume | ||
| L30-4C | The chamber accommodates either two （2） or four （4） samples and has an 80ml internal volume/td> |
Grip Options
The types of constructs used in ligament and tendon experiments vary widely and range from decellularized allografts to polymerized hydrogels. In order to handle these varied specimen types, the several options for gripping mechanisms are offered including:
| Clamp | Mechanical clamp vice grips with a collet locking mechanism. These grips are recommended for constructs which require a large clamping force. | ||
| In Situ Polymerization | These grips feature an integrated construct mold and porous grips to enable mechanical stimulation of hydrogel scaffolds. | ||
| Suture | A 316SS tab with suture holes drilled throughout accommodates wide constructs that will not fit within the clamp-type grips. | ||
| Custom Grips | Many constructs often require some level of grip customization. We have developed many grip designs and will adapt them to the your specific construct material and geometry. |
DynaGen® Stimulator:
The LigaGen bioreactor system includes the TC （tension/compression） mechanical stimulator. Featuring a 40N linear motor, the stimulator is lightweight, compact and incubator compatible. The TC stimulator controls both load and displacement and can be used with any of the LigaGen bioreactor chambers. The stimulator dimensions are as follows:
| L30-1X Dimensions: | 380mm（15"）H X 127mm（5"）W X 178mm（7"）D | ||
| Weight 2.9kg （6.5lb） | |||
| L30-4C Dimensions: | 330mm（13"）H X 102mm（4"）W X 102mm（4"）D | ||
| Weight 2.3kg （5.0lb） |
GrowthWorks® Software & Control:
A fifth generation design, the GrowthWorks Software and Control platform includes advanced capabilities, such as multiple waveform control, data acquisition and multi-motor operation. The intuitive software runs on a Windows® laptop computer and features a simple user interface. User defined stimulation profiles are controlled by the software and are readily monitored using graphical displays. Integrated data acquisition routines capture and record data at user prescribed intervals. The GrowthWorks Software is configurable for running four stimulators while simultaneously monitoring up to 16 transducers, allowing the researcher maximum flexibility.
The GrowthWorks Control Hardware features an embedded In CPU, integrated data acquisition and integrated motor drives. Communications between the laptop and control hardware is transmitted over a standard network cable. Primary advantages of the TGT Laptop/Controller approach are reduced physical size and easy upgradability. As future Windows versions or higher performance Laptops become available, either can be replaced without any software or controller changes. This ensures the GrowthWorks control system will be an integral part of your research for many years to come.
GrowthApps Stiffness Measurement & Adaptive Control:
The GrowthApps modules provided added capability to GrowthWorks. The addition of the Stiffness Measurement & Adaptive Control Application enables the system to make real-time measurements of the sample stiffness during culture. Described in U.S. Patent 7,410,792 B2 Instrumented bioreactor with material property measurement capability and process-based adjustment for conditioning tissue engineered medical products., the Application measures the mechanical response of the tissue construct as it is being stimulated and conditioned. The stiffness measurements can be used as guidelines for bench marking progress during culturing or for modifying the stimulation profile. Based upon the measured response, the bioreactor system is then capable of altering the stimulation profile. This technology enables researchers to automatically culture the tendon or ligament from initial seeding to an implantation-ready state with minimal operator intervention.
| LIGAMENT | ||
| Key: S=Standard, A=Available, - = not available | ||
| CHAMBERS | L30-1 | L30-4c |
| Individual ligament growth chamber | S | S |
| 6 parallel growth chambers, with up to 4 ligament constructs per chamber | - | A |
| Interface for tension grips | S | S |
| Perfusion and instrumentation ports per chamber | 4 | 4 |
| Bellows and seal to protect construct from outer environment | S | S |
| In-situ hydrogel seeding configuration: wire grips and casting mold | A | A |
| Patent pending grip lock during sample handling | S | S |
| Mechanical clamp grips | A | A |
| Inflatable clamp grips | A | A |
| STIMULATION | ||
| Axial stimulator and reaction frame | S | S |
| CONTROLS | ||
| Controller software, computer, and monitor | S | S |
| Power supplies and cabling | S | S |
| Automatic stimulation positioner | - | A |
| Mean flow control | A | A |
| Dynamic flow control | A | A |
| TISSUE MONITORING | ||
| Non-contact radial or axial distension measurement | A | A |
| Non-contact flow sensors | A | A |
| Catheter pressure transducers | A | A |
| ACCESSORIES | ||
| Media reservoir | S | S |
| Stimulator positioner （automatic） | - | A |
| Stimulator positioner （manual） | - | A |
| Incubator | A | A |
| BENEFITS | ||
| All-autoclavable components | S | S |
| Excellent sample visibility | S | S |
| Medium change without opening chamber via luer injection port fittings | S | S |
| Increased sterility with fast and clean sample change and access | S | S |
| Lightweight design for easy handling | S | S |
| Easily cleaned surface finishes | S | S |
| Transparent opposing reactor walls for optical inspection or measurement | S | S |
| Compact design to fit in standard incubators | S | S |
| * satisfaction guaranteed warranty | S | S |
Click here to download a LigaGen Brochure （Adobe PDF 120kb）