96-roundwell microplate 300 rpm

96-roundwell microplate 300 rpm

96圓井微板孔內(nèi)流體力學(xué)

對(duì)于96個(gè)圓孔板,，使用0.1至0.2毫升的培養(yǎng)體積,，在50毫米振幅的振動(dòng)篩上以300轉(zhuǎn)/分的速度進(jìn)行培養(yǎng),。這種振動(dòng)條件導(dǎo)致氧傳輸速率為20-40mmol/O2/L/h（對(duì)應(yīng)于100-200 H-1的KLA值）,。對(duì)于良好的混合，我們建議使用不超過(guò)0.15毫升培養(yǎng)體積（或者如果需要更高的氧傳輸速率甚至更少）,。如此低的培養(yǎng)體積使離心后的上清液很難取出,。一個(gè)更容易做到這一點(diǎn)的技巧是在離心之前加入等量的水或緩沖液（例如0.15ml ul）。請(qǐng)注意,，在搖動(dòng)條件下，你幾乎沒(méi)有空間進(jìn)行操作：需要小2.5g的離心力（如50 mm/300 rpm）來(lái)克服表面張力,，這樣就可以*混合到底部,，防止“死區(qū)”,。

50毫米振動(dòng)篩振幅•300轉(zhuǎn)/分

Hydrodynamics inside wells of a 96-roundwell microplate
For 96-roundwell plates, culture volumes between 0.1  and 0.2  ml are used, preferably at 300 rpm on a shaker with a 50 mm amplitude. This shaking condition leads to oxygen-transfer rates of 20-40 mmol O2/L/h (corresponding to kLa values of 100-200 h-1).For a good mixing, we advice to use no more than 0.15 ml culture volume (or even less if higher oxygen-transfer rates are needed). Such low culture volumes make it difficult to take off the supernatant after centrifugation. A trick to make this easier is to add an equal volume (so e.g. 0.15 ml ul) of water or buffer prior to centrifugation.Please note that you have little room to manouvre with regard to the shaking conditions: a centrifugal force in the order of minimally 2.5 g (as with 50 mm/300 rpm) is needed to overcome the surface tension, and so to obtain full mixing down to the bottom of the culture, and prevent “ dead zones”
50 mm shaker amplitude•300 rpm

 注：楊清辰發(fā)布