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| 供貨周期 | 現(xiàn)貨 | 應(yīng)用領(lǐng)域 | 醫(yī)療衛(wèi)生,生物產(chǎn)業(yè) |
|---|
詳細(xì)介紹
干細(xì)胞不同基底硬度機(jī)械力培養(yǎng)系統(tǒng)
特別適合細(xì)胞,、干細(xì)胞不同基底硬度牽張拉伸刺激培養(yǎng)或靜態(tài)培養(yǎng)（模量剛度范圍1-80 kPa）。
美國(guó)flexcell提供CellSoft™型號(hào)的不同基底硬度培養(yǎng)耗材包括：不同基底硬度牽張拉伸培養(yǎng)板,、培養(yǎng)皿和可用于顯微觀察的腔室載玻片（圓形多孔板）多種不同的種類,，該cellsoft不同基底硬度培養(yǎng)耗材共價(jià)包被Collagen I或其他蛋白配合flexcell細(xì)胞牽張拉伸培養(yǎng)儀，可對(duì)細(xì)胞進(jìn)行靜態(tài)或動(dòng)態(tài)牽拉應(yīng)力刺激,。更重要的一點(diǎn),，新型的CellSoft™ 培養(yǎng)板可以反復(fù)胰酶消化和再接種細(xì)胞，蛋白包被的表面可以重復(fù)使用多達(dá)三次,。
美國(guó)Flexcell公司專注于細(xì)胞組織應(yīng)力（牽張拉伸應(yīng)力,、三維水凝膠牽張拉伸應(yīng)力、壓應(yīng)力和流體切應(yīng)力等）加載刺激培養(yǎng)產(chǎn)品的設(shè)計(jì)和制造,，提供*的體外細(xì)胞拉應(yīng)力,、壓應(yīng)力和流體剪切應(yīng)力加載刺激與立體水凝膠支架三維細(xì)胞組織牽拉加載培養(yǎng)系統(tǒng)而*,。
其產(chǎn)品成熟度高,、成功應(yīng)用文獻(xiàn)量達(dá)4000多篇，國(guó)內(nèi)有包括上海交通大學(xué)、復(fù)旦大學(xué),、同濟(jì)大學(xué),、上海第九醫(yī)院、中科院力學(xué)所,、北京大學(xué)第三醫(yī)院,、北航生物與醫(yī)學(xué)工程學(xué)院、都醫(yī)科大學(xué),、廣州醫(yī)科大學(xué),、南方科技大學(xué)、福建協(xié)和醫(yī)院,、南方醫(yī)科大學(xué)近100家成功高校,、醫(yī)院及基礎(chǔ)科研單位使用，無(wú)技術(shù)風(fēng)險(xiǎn)和使用風(fēng)險(xiǎn),，flexcell體外高通量細(xì)胞牽張拉伸力,、壓應(yīng)力以及流體剪切力加載培養(yǎng)系統(tǒng)已成為細(xì)胞力學(xué)體外加載模型的黃金標(biāo)準(zhǔn)，是細(xì)胞組織力學(xué)研究者的shou選,。
FX-5000T細(xì)胞牽張拉伸應(yīng)力加載系統(tǒng)(Flexcell FX5000 Tension system)
系統(tǒng)基本原理（負(fù)氣壓交換模式）：
橡膠密封墊在細(xì)胞培養(yǎng)板基底膜與基板之間形成封閉腔,，把此密封腔的進(jìn)、出氣管插入二氧化碳培養(yǎng)箱里,，把此密封腔放入二氧化碳培養(yǎng)箱,， 利用封閉腔抽真空產(chǎn)生的負(fù)壓使彈性基底膜（拉動(dòng)三維支架）發(fā)生形變，通過(guò)計(jì)算機(jī)控制系統(tǒng)調(diào)節(jié)氣體的壓力來(lái)改變基底膜的形變量,進(jìn)而使貼壁生長(zhǎng)的細(xì)胞受到牽拉加載刺激,。
亮點(diǎn)：
1）該系統(tǒng)對(duì)二維,、三維細(xì)胞和組織各種培養(yǎng)物提供軸向和圓周應(yīng)力加載；不但具有雙軸向拉伸力加載,，還具備單軸向加力功能
2）計(jì)算機(jī)控制的應(yīng)力加載系統(tǒng),，為體外培育的細(xì)胞提供的、可控制的,、可重復(fù)的,、靜態(tài)的或者周期性的應(yīng)力變化。
3）使用真空泵,，抻拉培養(yǎng)板底部的彈性硅膠模,，細(xì)胞培養(yǎng)板底部伸展度可達(dá)到33%，通過(guò)氣體裝置可以自動(dòng)調(diào)節(jié)和控制應(yīng)力,。
4）基于柔性膜基底變形,、受力均勻;
5）可實(shí)時(shí)觀察細(xì)胞、組織在應(yīng)力作用下的反應(yīng);
6）*的flexstop隔離閥可使同一塊培養(yǎng)板力的一部分培養(yǎng)孔的細(xì)胞受力,，一部分培養(yǎng)孔的細(xì)胞不受力,，方便對(duì)比實(shí)驗(yàn);
7）與壓力傳導(dǎo)儀整合,，同時(shí)兼?zhèn)涠嗤ǖ兰?xì)胞壓力加載功能;
8）與Flex Flow平行板流室配套，可在牽拉細(xì)胞的同時(shí)施加流體切應(yīng)力;
9）多達(dá)4通道,，可4個(gè)不同程序同時(shí)運(yùn)行,，進(jìn)行多個(gè)不同拉伸形變率對(duì)比實(shí)驗(yàn)；
10）同一程序中可以運(yùn)行多種頻率,，多種振幅和多種波形,；
11）加載模擬波形種類豐富：靜態(tài)波形、正旋波形,、心動(dòng)波形,、三角波形、矩形以及各種特制波形,；
12）更好地控制在超低或超高應(yīng)力下的波形,；
13)電腦系統(tǒng)對(duì)牽張拉伸力加載周期、大小,、頻率,、持續(xù)時(shí)間智能調(diào)控
14)加載分析各種細(xì)胞在牽張拉應(yīng)力刺激下的生物化學(xué)反應(yīng)
15)伸展度范圍廣：0-33%
16)牽拉頻率范圍廣：0.01-5Hz
17)典型應(yīng)用:
該系統(tǒng)感應(yīng)各種細(xì)胞在應(yīng)力刺激下的生物化學(xué)反應(yīng)，例如：骨骼細(xì)胞,，肺細(xì)胞,，心肌細(xì)胞，血細(xì)胞,，皮膚細(xì)胞,，
肌腱細(xì)胞，韌帶細(xì)胞,，軟骨細(xì)胞和骨細(xì)胞等各種2D或3D細(xì)胞組織,。
典型應(yīng)用科室:
| 口腔 | 顳下頜關(guān)節(jié)滑膜細(xì)胞、人牙周膜細(xì)胞,、口腔上皮細(xì)胞,、口腔鱗癌KB細(xì)胞等 |
| 骨: | 骨骼細(xì)胞、肌腱細(xì)胞,、韌帶細(xì)胞,、軟骨細(xì)胞和骨細(xì)胞、骨髓間充質(zhì)干細(xì)胞,， 軟骨組織,、椎間盤骨組織、肌腱組織,、韌帶組織等 |
| 肺呼吸 | 肺細(xì)胞,、肺上皮細(xì)胞、肺動(dòng)脈內(nèi)皮細(xì)胞,、人肺微血管內(nèi)皮細(xì)胞 |
| 眼科視覺(jué)神經(jīng) | 眼上皮細(xì)胞,、眼小梁組織細(xì)胞,、視網(wǎng)膜神經(jīng)細(xì)胞 |
| 心血管/高血壓: | 心肌細(xì)胞、血細(xì)胞,、心血管平滑肌細(xì)胞,、血管內(nèi)皮細(xì)胞 |
| 生殖 | 腎膀胱細(xì)胞,、平滑肌細(xì)胞/尿路上皮及尿路上皮細(xì)胞,、腎小管上皮細(xì)胞 |
| 消化 | 腸上皮細(xì)胞、 胃上皮細(xì)胞,、胃血管內(nèi)皮細(xì)胞 |
| 皮膚 | 皮膚細(xì)胞,、皮膚成纖維細(xì)胞 |
2、FX-5000C細(xì)胞壓力加載系統(tǒng)(flexcell FX5000 Compression system)——提供樣機(jī)體驗(yàn)
系統(tǒng)基本原理（正氣壓交換模式）：
利用橡膠密封墊在細(xì)胞培養(yǎng)板基底膜與基座之間形成封閉腔,，把此密封腔的進(jìn),、出氣管插入二氧化碳培養(yǎng)箱里，把此密封腔放入二氧化碳培養(yǎng)箱,，利用封閉腔正氣壓擠壓培養(yǎng)孔里的活塞,，進(jìn)而使活塞和固定臺(tái)之間的凝膠三維培養(yǎng)物間接受到壓力發(fā)生形變，通過(guò)計(jì)算機(jī)控制系統(tǒng)調(diào)節(jié)氣體的壓力來(lái)改變基底膜的形變量,。
(注釋：壓力加載培養(yǎng)板每個(gè)培養(yǎng)孔里都有一對(duì)活塞或固定臺(tái))
亮點(diǎn)
1）該系統(tǒng)對(duì)各種組織,、三維細(xì)胞培養(yǎng)物提供周期性或靜態(tài)的壓力加載；
2）基于柔性膜基底變形,、受力均勻;
3）可實(shí)時(shí)觀察細(xì)胞,、組織在壓力作用下的反應(yīng);
4）可有選擇性地封阻對(duì)細(xì)胞的應(yīng)力加載;
5）同時(shí)兼?zhèn)涠嗤ǖ兰?xì)胞牽拉力加載功能;
6）多達(dá)4通道，可4個(gè)不同程序同時(shí)運(yùn)行,，進(jìn)行多個(gè)不同壓力形變率對(duì)比實(shí)驗(yàn),；
7）同一程序中可以運(yùn)行多種頻率(0.01- 5 Hz)，多種振幅和多種波形,；
8）更好地控制在超低或超高應(yīng)力下的波形,；
9）多種波形種類：靜態(tài)波形、正旋波形,、心動(dòng)波形,、三角波形、矩形以及各種特制波形,；
10)電腦系統(tǒng)對(duì)壓力加載周期,、大小、頻率,、持續(xù)時(shí)間智能調(diào)控
11)壓力范圍：0.1 - 14磅,，夾在活塞和固定臺(tái)之間的BioPress細(xì)胞培養(yǎng)板可承受正壓力0.1---14磅
12)典型應(yīng)用科室:
檢測(cè)各種三維細(xì)胞組織在壓力作用下的生物變化、反應(yīng),，
例如：軟骨組織,，椎間盤骨組織,，肌腱組織，韌帶組織,，以及從肌肉,，肺，心臟,，血管,，皮膚，肌腱,，韌帶,，軟骨和骨中分離出來(lái)的細(xì)胞。
13)在智能電腦主機(jī)的控制下,，壓力傳導(dǎo)儀內(nèi)的密封閥門裝置自動(dòng)調(diào)節(jié)和控制壓力,。
14)系統(tǒng)具有模塊化易升級(jí)，可擴(kuò)展拉應(yīng)力加載,、流利切應(yīng)力加載,、三維細(xì)胞組織培養(yǎng)功能。具有細(xì)胞組織力學(xué)所要求的所有類型：牽張拉伸力,、壓力,、流體切應(yīng)力加載刺激功能。
15)通過(guò)StagePress顯微壓應(yīng)力加載設(shè)備,實(shí)時(shí)觀察細(xì)胞,、組織在拉/壓應(yīng)力作用下的反應(yīng)
3,、全自動(dòng)可牽張拉伸刺激立體水凝膠支架三維細(xì)胞培養(yǎng)系統(tǒng)(Flexcell TissueTrain System)——提供樣機(jī)體驗(yàn)
| FLEXCELL Tissue Train®是個(gè)獨(dú)立的全自動(dòng)細(xì)胞組織三維培養(yǎng)、組織構(gòu)建計(jì)算機(jī)智能控制的生物反應(yīng)器系統(tǒng),，它允許研究者創(chuàng)建三維基質(zhì)凝膠支架,， |
| 真正意義上的三維培養(yǎng)——該系統(tǒng)以多種包被表面(Amino、Collagen (Type I or IV),、Elastin,、 ProNectin (RGD)、Laminin (YIGSR))的水凝膠為細(xì)胞外基質(zhì)支架——水凝膠支架因在液態(tài)時(shí)包裹細(xì)胞,，固態(tài)時(shí)形成交聯(lián)網(wǎng)絡(luò),，細(xì)胞粘附力強(qiáng),良好水分、養(yǎng)分交換,。 水凝膠是一種狀似果凍的物質(zhì),，具有高彈性、吸水性的聚合物組成的網(wǎng)狀物,，用于組織工程中,，作為幫助細(xì)胞生長(zhǎng)和發(fā)展的支架. 利用立體水凝膠支架作為平臺(tái)，觀察不同細(xì)胞的交互作用，建立組織和器官,。同時(shí)通過(guò)在立體環(huán)境中培育細(xì)胞,，有助于更深入地了解細(xì)胞過(guò)程和交互作用. 在基質(zhì)里細(xì)胞培養(yǎng)、構(gòu)建生物組織,，可為三維細(xì)胞,、組織提供雙軸向應(yīng)力和單軸向應(yīng)力，F(xiàn)LEXCELL Tissue Train® |
是當(dāng)今科研界的可拉伸刺激三維細(xì)胞培養(yǎng),、生物組織構(gòu)建系統(tǒng),。 4\多流場(chǎng)六通道流體切應(yīng)力培養(yǎng)與實(shí)時(shí)觀察系統(tǒng)
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二,、excellness代理,PrimeCoat彈性基底培養(yǎng)皿板
PrimeCoat series
The ExCellness PrimeCoat series is designed specifically to provide a biomimetic cell culture environment that improves cell characteristics and phenotype in laboratory applications.
Key features
- PrimeCoat elastic substrates are easy to use for cell culture and subsequent analysis.
- PrimeCoat elastic substrates are available with 6 degrees of softness within the elasticity range of body tissues: 2, 5, 10, 15, 30 and 100 kPa.
- PrimeCoat elastic substrates are available in 4 standard formats: 100 mm diameter dishes, 40 mm diameter dishes, 24-well plates and 20x20 mm cover slips.
- PrimeCoat elastic substrates require to be coated by the end user to promote cell adhesion.
- PrimeCoat elastic substrates are transparent. Cells can be visualized with standard transmission light microscopes (e.g., Phase contrast, DIC).
- PrimeCoat elastic substrates are compatible with most standard molecular or cellular techniques: immunofluorescence, immunohistochemistry, protein analysis (e.g., Western blotting, and RNA/DNA extraction).
Biomimetic cell culture substrates at reach
PrimeCoat series combines simplicity and accessibility:
- simplicity of cell handling
- straightforward compatibility with cell analysis tools
- accessible prices
ExCellness in selected peer reviewed publications
In the following peer-reviewed publications, ExCellness biomimetic cell culture devices have been used or cited:
- A Glentis et al. Cancer-associated fibroblasts induce metalloprotease-independent cancer cell invasion of the basement membrane. Nat Commun. 2017 Oct 13;8(1):924.
- N Vedrenne et al. Isolation of astrocytes displaying myofibroblast properties and present in multiple sclerosis lesions. Neurochem. Res. 2017 Apr 22.
- X L Chen et al. MicroRNA-21 preserves the fibrotic mechanical memory of mesenchymal stem cells. Nature Materials 16, 379–389 (2017).
- MR Zeglinski et al. Chronic expression of Ski induces apoptosis and represses autophagy in cardiac myofibroblasts. Biochim. Biophys. Acta. 2016 Jun;1863(6 Pt A):1261-8.
- H Chen et al. Mechanosensing by the α6-integrin confers an invasive fibroblast phenotype and mediates lung fibrosis. Nat. Commun. 2016 Aug 18;7: 12564.
- NP Talele et al. Expression of α-Smooth Muscle Actin Determines the Fate of Mesenchymal Stromal Cells. Stem Cell Reports. 2015 Jun 9;4(6) : 1016-30.
- V Sarrazy et al. Integrins avb5 and avb3 promote latent TGF-beta 1 activation by human cardiac fibroblast contraction. Cardiovasc Res (2014) 102 (3)
- VF Achterberg et al. The nano-scale mechanical properties of the extracellular matrix regulate dermal fibroblast function.J Invest Dermatol. 2014 Jul;134(7):1862-72.
- JA Cadby et al. Differences between the Cell Populations from the Peritenon and the Tendon Core with Regard to Their Potential Implication in Tendon Repair. 2014. PLoS ONE 9(3): e92474.
- T. Grand et al. Aggravation of Cardiac Myofibroblast Arrhythmogeneicity by Mechanical Stress. Cardiovasc Res. 2014 Dec 1;104(3):489-500.
- JA Cadby. Can we improve tendon healing in the horse? A multi-angle study of a multi-facet problem. ISBN: 978-90-5335-715-6.
- A Vashist et al. Recent advances in hydrogel based drug delivery systems for the human body. J. Mater. Chem. B, 2014,2, 147-166.
- EP van der Veer et al. The RNA-Binding Protein Quaking is a Critical Regulator of Vascular Smooth Muscle Cell Phenotype. Circ Res. 2013 Oct 12;113(9):1065-75.
- A De Boeck et al. Differential secretome analysis of cancer-associated fibrobroblasts and bone marrow-derived precursors toidentify microenvironmental regulators of colon cancerprogression. Proteomics 2013,13,379-388.
- C Godbout et al. The Mechanical Environment Modulates Intracellular Calcium Oscillation Activities of Myofibroblasts.PLoS One. 2013; 8(5): e64560.
- S Constant et al. Colon Cancer: Current Treatments and Preclinical Models for the Discovery and Development of New Therapies. Drug discovery; Editor Hany A. El-Shemy. ISBN 978-953-51-0906-8.
- JL Balestrini et al. The mechanical memory of lung myofibroblasts. Integr. Biol., 2012,4, 410-421.
- Stem Cells and Cancer Stem Cells, Volume 8. Therapeutic Applications in Disease and Injury. Editors M.A. Hayat. ISBN 978-94-007-4797-5.
- A Skardal et al. Bioprinted amniotic fluid-derived stem cells accelerate healing of large skin wounds. Stem Cells Transl Med. 2012 Nov;1(11):792-802. doi: 10.5966/sctm.2012-0088. Epub 2012 Oct 29.
- A Skardal et al. Substrate elasticity controls cell proliferation, surface marker expression and motile phenotype in amniotic fluid-derived stem cells. J Mech Behav Biomed Mater. 2013 January; 17: 307-316.
- BJ Crider et al. Myocardin-Related Transcription Factors A and B Are Key Regulators of TGF-b1-Induced Fibroblast to Myofibroblast Differentiation. Journal of Investigative Dermatology (2011) 131, 2378-2385.
- L Follonier Casla et al. A new lock-step mechanism of matrix remodelling based on subcellular contractile events. Journal of Cell Science 123, 1751-1760.
訂貨信息：
Dishes
Product Code Description
01. 100.002.00 2 kPa culture surface 100 mm ø
01. 100.005.00 5 kPa culture surface 100 mm ø
01. 100.010.00 10 kPa culture surface 100 mm ø
01. 100.015.00 15 kPa culture surface 100 mm ø
01. 100.030.00 30 kPa culture surface 100 mm ø
01. 100.100.00 100 kPa culture surface 100 mm ø
Product Code Description
01. 035.002.00 2 kPa culture surface 35 mm ø
01. 035.005.00 5 kPa culture surface 35 mm ø
01. 035.010.00 10 kPa culture surface 35 mm ø
01. 035.015.00 15 kPa culture surface 35 mm ø
01. 035.030.00 30 kPa culture surface 35 mm ø
01. 035.100.00 100 kPa culture surface 35 mm ø
Multi-well plates
Product Code Description
04.024.002.00 2 kPa culture surface 24-well plate
04.024.005.00 5 kPa culture surface 24-well plate
04.024.010.00 10 kPa culture surface 24-well plate
04.024.015.00 15 kPa culture surface 24-well plate
04.024.030.00 30 kPa culture surface 24-well plate
04.024.100.00 100 kPa culture surface 24-well plate
Cover slips
Product Code Description
03.020.002.00 2 kPa culture surface 20x20 mm c.slip
03.020.005.00 5 kPa culture surface 20x20 mm c.slip
03.020.010.00 10 kPa culture surface 20x20 mm c.slip
03.020.015.00 15 kPa culture surface 20x20 mm c.slip
03.020.030.00 30 kPa culture surface 20x20 mm c.slip
化工儀器網(wǎng)