日韩av大片在线观看欧美成人不卡|午夜先锋看片|中国女人18毛片水多|免费xx高潮喷水|国产大片美女av|丰满老熟妇好大bbbbbbbbbbb|人妻上司四区|japanese人妻少妇乱中文|少妇做爰喷水高潮受不了|美女人妻被颜射的视频,亚洲国产精品久久艾草一,俄罗斯6一一11萝裸体自慰,午夜三级理论在线观看无码

【文獻(xiàn)標(biāo)題】Sphk1 participates in malignant progression of breast cancer by regulating epithelial-mesenchymal transition and stem cell characteristics

【作者】Zhijie Chen, Bingxiong Liu

【作者單位】漢川市人民醫(yī)院（Hanchuan People’s Hospital）

【文獻(xiàn)中引用產(chǎn)品】

霍亂毒素 CAS號(hào)：9012-63-9

【關(guān)鍵詞】Breast cancer,，Sphingosine kinase 1,，Epithelial-mesenchymal transition,，Stem cell，Metastasis

【DOI】doi.org/10.1016/j.tice.2020.101380

【影響因子(IF)】2.90

【出版期刊】《Tissue and Cell》

【產(chǎn)品原文引用】

Cell and culture

Human breast epithelial cells (MCF-10A, CRL-10317) and BC cell lines MCF-7 (CRL-3435), T47D (HTB-133), SKBR3 (HTB-30), MDA-MB-231 (HTB-26), BT-474 (HTB-20) were purchased from American Tissue Culture Collection. The cells, except MDA-MB-23 cells, were cultured in DMEM (12491015, ThermoFisher, USA) containing 10 % FBS (30067334, ThermoFisher, USA), 1% penicillin/streptomycin (15140163,ThermoFisher, USA) at 37 °C in 5% CO2. MCF-10A cell line was cultured with additional 100 ng/mL cholera toxin (Hengyuan,China, hyswbio) on the basis of other cell culture condition. After culture for one day, the original medium was discarded, and the cells were washed 1–2 times by PBS. Next, 5 mL fresh DMEM medium was added and further incubated with the cells in a CO2 incubator. When the confluence reached 80%–90%, the cells were subcultured. MDA-MB-231 cells were cultured in Leibovitz’s L15 medium (11415064, ThermoFisher, USA) without CO2,。

完整版PDF文獻(xiàn)請(qǐng)咨詢?cè)诰€客服或者聯(lián)系我司業(yè)務(wù)員,！

更多公司福利請(qǐng)關(guān)注“恒遠(yuǎn)生物”VX公眾號(hào)！