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15
SNU-449細(xì)胞,， 人肝癌細(xì)胞(ATCC® CRL-2234™)
| Permits and Restrictions | View Restrictions |
|---|---|
| Organism | Homo sapiens, human |
| Tissue | liver |
| Product Format | frozen |
| Morphology | epithelial; diffusely spreading cells |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain Hepatitis B virus] |
| Disease | grade II-III/IV,hepatocellular carcinoma |
| Age | 52 years |
| Gender | male |
| Ethnicity | Asian |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | aneuploid; modal number = 57 |
|---|---|
| Derivation | SNU-449 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient prior to cytotoxic therapy. Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum. |
| Clinical Data | 52 years Asian male SNU-449細(xì)胞， 人肝癌細(xì)胞 |
| Comments | Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed. Grossly, the original tumor was single nodular with perinodular extensions. Histologically, it was predominantly compact and minor trabecular type. The cultured cells contain a single or double nucleus. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing | Protocol:
Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
