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【文獻(xiàn)標(biāo)題】Reparative effects of lycium barbarum polysaccharide on mouse ovarian injuries induced by repeated superovulation

【作者】Bing Liu,，Jun-Li Wang,，Xin-Mei Wang,，et.al

【作者單位】北京農(nóng)業(yè)大學(xué)（Beijing University of Agriculture）

【文獻(xiàn)中引用產(chǎn)品】

小鼠8羥基脫氧鳥苷（8-OHdG）ELISA試劑盒

小鼠過氧化脂質(zhì)（LPO）ELISA試劑盒

【關(guān)鍵詞】Superovulatory times，Injury model,，LBP,，Remedy，Oxidative stress

【DOI】doi.org/10.1016/j.theriogenology.2020.01.048

【出版期刊】《Theriogenology》

【產(chǎn)品原文引用】

8-OHdG and LPO levels of the collected ovarian tissues were determined using an ELISA kit, according to the manu-facturer’s instructions (Shanghai Hengyuan Biotechnology Co., Ltd,Shanghai, China). The collected mouse ovaries were cracked with lysate. Each 10 mL henylmethane-sulfonyl (PMSF) was added in 1 mL radio immuno precipitation assay (RIPA) as lysate. Every 1 g of tissue with 9 mL lysate, then coated with mouse antibody against 8-OHdG or LPO. After incubation and washing concentrations of 8-OHdG or LPO were determined as described previously .Intra-and inter-CVs: 8-OHdG (6.3%, 8.1%), LPO (5.2%, 7.4%).