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Clontech AH109說明書,，AH109說明書，Clontech AH109
AH109
| 型號 | 載體名稱 | 出品公司 | 載體用途 |
| VNC0491 | AH109 | Clontech | 配套釀酒酵母 |
his section provides detailed phenotypes of the yeast strains included with MATCHMAKER System 3
and MATCHMAKER GAL4 cDNA and Genomic Libraries. For additional information on the growth and
maintenance of yeast, see the YPH, Chapter III. We also recommend Guthrie & Fink’s Guide to Yeast
Genetics and Molecular Biology （1991） and Heslot & Gailardin’s Molecular Biology and Genetic
Engineering of Yeasts （1992）.
A. Yeast Host Strains
The complete genotypes of AH109, Y187, and CG-1945 are provided in Table II. All strains are
gal4
–
and gal80
–
; this prevents interference of native regulatory proteins with the regulatory
elements in the two-hybrid system.
1. Use AH109 as the host strain if you plan to screen an AD/library using HIS3, ADE2, and MEL1.
2. System 3 Users Only: Use Y187 as the host strain if you plan to test for an interaction between
two known proteins using the lacZ reporter only. In addition, use Y187 as a mating partner to
verify protein interactions.
3. Library Users Only: Use CG-1945 as the host strain if you plan to separate DNA/bait and
AD/library plasmids by cycloheximide counterselection. Alternatively, use pGBKT7 to construct your bait. This DNA-BD vector contains a kanamycin resistance marker; therefore,
vectors can be separated in E. coli without cycloheximide counterselection.
