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Matchmaker™ Library Construction & Screening Kits (pGBKT7,,pGADT7,,pGBKT7-53,pGADT7-T,,pGBKT7-Lam)
2013-7-23 閱讀(6613)
pGBKT7-53
Bacteria Resistance:KanamycinMammalian Selection:-
載體特點:與pGADT7-T構(gòu)成Matchmaker Two-Hybrid System 3的雙雜交正對照,。
pGBKT7-53 encodes a fusion of the murine p53 protein (a.a. 72–390) and the GAL4 DNA-BD (a.a. 1–147). The murine p53 cDNA (GenBank Accession #K01700) was cloned into pGBKT7 at the EcoR I and BamH I sites. The p53 insert was derived from the plasmid described in Iwabuchi et al. (1993); plasmid modification was performed at Clontech. pGBKT7-53 has not been sequenced.

pGBKT7-Lam
Bacteria Resistance:KanamycinMammalian Selection:-
載體特點:Matchmaker Two-Hybrid System 3的負(fù)對照。
pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bar et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.
