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ANGPTL3 Human
  • ANGPTL3 Human

貨物所在地：福建廈門市

更新時間：2024-11-24 21:00:06

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ANGPTL3 Human
產(chǎn)品編號：CYT-248
產(chǎn)品規(guī)格：2μg/10μg/1mg

Synonyms Angiopoietin 5, ANGPT5, ANGPTL3, Angiopoietin like Protein 3.
Introduction ANGPTL3 and ANGPTL4 are angiopoietin-like proteins secreted and expressed mainly by the liver, their role being the regulation of triglyceride metabolism by inhibiting the lipolysis of triglyceride-rich lipoproteins. During different nutritional states （feeding/fasting） the levels of the circulating triglycerides are regulated by Angptl3 and Angptl4 through differential inhibition of Lipoprotein lipase （LPL） as shown by the experimental data. The molecular structure of ANGPTL3 is similar to that of the angiopoietins （vascular endothelial growth factors）. Deletion mutants of human Angiopoietin 5 were used in order to demonstrate that the N-terminal domain （fragment 17-207） and not the C-terminal fibrinogen-like domain （fragment 207-460） increased the plasma triglyceride levels in mice.
Description The ANGPTL3 Human Recombinant is produced with N-terminal fusion of His-Tag.
The Angiopoietin-like protein 3 His Tagged Fusion Protein is 26kDa containing 207 amino acid residues of the ANGPTL3 Human and 16 additional amino acid residues – His-Tag （underlined）.
MRGSHHHHHH GMASHMSRID QDNSSFDSLS PEPKSRFAML DDVKILANGL LQLGHGLKDF VHKTKGQIND IFQKLNIFDQ SFYDLSLQTS EIKEEEKELR RTTYKLQVKN EEVKNMSLEL NSKLESLLEE KILLKVKY LEEQLTNLIQ NQPETPEHPE VTSLKTFVEK QDNSIKDLLQ TVEDQYKQLN HSQIKEIE NQLRRTSIQE PTEISLSSKP RAP.
Source Escherichia Coli.
Specificity The amino acid sequence of the recombinant is * homologous to the 17-223 amino acid sequence of the Human Angiopoietin-like proteins-3 precursor without signal sequence.
Purification Method One-step procedure using affinity Ni-NTA chromatography.
Formulation Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.
Purity Angiopoietin 5 purity is greater than 95% as determined by SDS-PAGE.
Solubility Add 0.2 ml of 0.1M Acetate buffer pH-4 and let the lyophilized pellet of dissolve compley. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of Angiopoietin 5 is limited.
Stability Store lyophilized at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Angiopoietin 5 can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized Angiopoietin 5 remains stable until the expiry date when stored at -20°C.
Applications Western blotting, ELISA.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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