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BMP 2 Human
  • BMP 2 Human

貨物所在地：福建廈門市

更新時(shí)間：2024-11-24 21:00:06

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BMP 2 Human
產(chǎn)品編號(hào)：CYT-261
產(chǎn)品規(guī)格：2μg/10μg/1mg

Catalogue Number CYT-261
Synonyms BMP-2, BMP2A.
Introduction BMP2 belongs to the transforming growth factor-beta （TGFB） superfamily. Bone morphogenic protein induces bone formation. BMP2 is a candidate gene for the autosomal dominant disease of fibrodysplasia （myositis） ossificans progressiva.
Description Bone Morphogenetic Protein-2 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 115 amino acids and having a molecular mass of 26018 Dalton.
The BMP-2 is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation BMP-2 was lyophilized from a concentrated （1mg/ml） sterile solution containing 20mM NaAc pH=4.
Solubility It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-2 in sterile 20mM AcOH （acetic Acid） not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Bone Morphogenetic Protein-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE.
Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Ala-Lys-His.
Biological Activity 1. The ED50 as determined by the cytolysis of MC3T3-E1 cells is < 50 ng/ml. 2. When implanted in the mouse thighbone muscle and grow for 14 days, Muscle tissue was harvested and examined biochemically for calcium uptake. Our unit defined as 1 microgram calcium absorbed. The specific activity is more than 1000 Units/mg. 3. The activity of BMP-2 was tested using alkaline phosphatase, the activity in mouse muscle myoblast C2C12 cell line was found to be > 1 x 100,000 U/mg protein. 4. The ectopic osteogenesis capacity is 1000mg fresh bone per 1mg of BMP-2.
Protein content Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.

2. Analysis by RP-HPLC, using a standard solution of BMP-2 as a Reference Standard.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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