ELISA試劑盒試驗原理：
試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知AOPP濃度的標準品,、未知濃度的樣品加入微孔酶標板內(nèi)進行檢測,。先將AOPP和生物素標記的抗體同時溫育。洗滌后,，加入親和素標記過的HRP,。再經(jīng)過溫育和洗滌，去除未結(jié)合的酶結(jié)合物,，然后加入底物A,、B，和酶結(jié)合物同時作用,。產(chǎn)生顏色,。顏色的深淺和樣品中AOPP的濃度呈比例關(guān)系。
英文名稱  Anti-BRF1/RNA polymerase III 
中文名稱  RNA聚合酶III抗體 
別    名  B related factor 1; B-related factor 1; BRF; BRF-1; brf1; brfu; General transcription factor IIIB, 90kD subunit; GTF3B; hBRF; hTFIIIB90; RNA polymerase III; subunit 2; TAF3B2; TAF3C; TATA box-binding protein-associated factor; TF3B_HUMAN; TFIIIB90; Transcription factor IIIB 90 kDa subunit; Transcription initiation factor IIIB. 
濃    度  1mg/1ml 
規(guī) 格  0.2ml/200μg  1ml/1mg
抗體來源  Rabbit  
克隆類型  polyclonal 
交叉反應(yīng)  Human, Mouse, Rat   
產(chǎn)品類型  一抗    
研究領(lǐng)域  細胞生物 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 表觀遺傳學(xué)  
蛋白分子量  predicted molecular weight: 74kDa 
性    狀  Lyophilized or Liquid 
免 疫 原  KLH conjugated synthetic peptide derived from Human BRF1/RNA polymerase III 
亞    型  IgG 
純化方法  affinity purified by Protein A 
儲 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 
產(chǎn)品應(yīng)用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 
（石蠟切片需做抗原修復(fù)） 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
ELISA試劑盒RNA聚合酶III抗體產(chǎn)品介紹 responsible for the synthesis of a different class of RNA. RNA polymerase I (Pol I) transcribes the rRNA (ribosomal RNA) genes for the precursor of the 28S, 18S, and 5.8S molecules of the ribosome. RNA polymerase II transcribes protein-encoding genes into mRNA (messenger RNA) and snRNA (small nuclear RNA) genes into snRNAs that influence the processing of other classes of RNA. RNA polymerase III (Pol III) transcribes the 5S rRNA genes and all of the tRNA (transfer RNA) genes. Each class of RNA polymerase is assembled from 9 to 15 different polypeptides. The RPB6 and RPB8 subunits are shared by all 3 RNA polymerases.
Function : General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
Subcellular Location : Nucleus.
Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity : Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.