P19小鼠睪丸畸胎瘤細(xì)胞(附鑒
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小鼠睪丸畸胎瘤細(xì)胞P19
種屬 | 小鼠 |
別稱 | P-19 |
組織來(lái)源 | 胚胎 |
疾病 | 畸胎癌 |
傳代比例/細(xì)胞消化 | 1:2傳代,,消化2-3分鐘 |
培養(yǎng)基配置 | MEM-a培養(yǎng)基,;2.5%胎牛血清;7.5 %小牛血清,;1%雙抗 |
簡(jiǎn)介 | P19細(xì)胞株是從C3H/He小鼠中誘導(dǎo)的惡性畸胎瘤中建立的,。該細(xì)胞在含有0.1mM2-巰乙醇的培養(yǎng)基中可高效率地 克隆。該細(xì)胞具有多能性,,在500nM誘導(dǎo)下可以分化成神經(jīng)和神經(jīng)膠質(zhì)樣細(xì)胞;在0.5%~1.0%二甲亞砜 (DMSO)存在下,,分化形成心臟和骨骼肌樣細(xì)胞,,但不形成神經(jīng)或神經(jīng)膠質(zhì)樣細(xì)胞;在DMSO和同時(shí)存在時(shí),, 細(xì)胞的分化與只有一樣。 |
形態(tài) | 上皮細(xì)胞樣 |
生長(zhǎng)特征 | 貼壁生長(zhǎng) |
倍增時(shí)間 | ~48-72h |
保藏機(jī)構(gòu) | ATCC;CRL-1825 |
mechanisms of response and resistance to various cancer treatments; however clinical challenges exist using current strategies. Significant questions remain regarding monitoring of archival versus fresh tissue, assessment of static versus dynamic markers, evaluation of limited tissue samples, and the translation of insights gained from immunologically hot tumors such as melanoma to other cold tumor microenvironments prevalent in other cancer types. Current and emerging