MCA-205 小鼠纖維肉瘤細(xì)胞
Catalogue No.: C867
Product Format: a T25 flask
Culture Properties：貼壁
Complete Growth Medium: 89%1640+10%FBS+1%雙抗
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Application: Cells and cancer research
NOTE: FOR RESEARCH USE ONLY.

Components

Operation steps for cell culture

1. 吸走用于培養(yǎng)基,，加入3-5mL PBS后輕輕晃動培養(yǎng)瓶潤洗細(xì)胞,；
2. 吸干凈PBS后加入1mL 0.25%-0.53mM EDTA，輕輕搖晃培養(yǎng)皿使浸沒細(xì)胞表面,，培養(yǎng)瓶放37度培養(yǎng)箱消化,；
   （細(xì)胞對于消化比較敏感，消化過度會嚴(yán)重影響細(xì)胞狀態(tài),，導(dǎo)致細(xì)胞傳代死亡漂浮或者傳代后不長,。細(xì)胞消化到細(xì)胞間隙變大但未脫落，可以輕輕吹下時即可終止,，禁止消化到細(xì)胞漂浮,。混勻細(xì)胞時盡量輕柔,，不要吹出大量氣泡,。）
3. 加入6-8ml培養(yǎng)基終止消化，輕輕吹下細(xì)胞混勻,；
4. 將混勻的細(xì)胞1000rpm（約150g）離心3min,，棄上清，再用新鮮培養(yǎng)基重懸37度培養(yǎng)箱繼續(xù)培養(yǎng),；

傳代比例: 不同細(xì)胞生長速度不一,，具體傳代比例視細(xì)胞生長速度而定，大部分細(xì)胞適用1:3-1:4傳代,，生長較慢的細(xì)胞可以1:2傳代,。

sensitivity and specificity were 94.50% and 90.00%，respectively. Conclusion The serum level ofFGF2 and TRPC1 inpatients with schizophrenia is significantly increased，and the serum levels of FGF2 and TRPC1 are closely related to the psychiatric symptoms of patients.