IPEC-J2 豬小腸上皮細(xì)胞
- 公司名稱 上海富雨生物科技有限公司
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產(chǎn)品名稱：IPEC-J2 豬小腸上皮細(xì)胞,、IPEC-J2 豬小腸上皮細(xì)胞,、IPEC-J2 豬小腸上皮細(xì)胞、IPEC-J2 豬小腸上皮細(xì)胞,；
豬正常腸上皮細(xì)胞IPEC-J2
| 種屬 | 豬 |
| 別稱 | IPECJ2; Intestinal Porcine Epithelial Cell line-J2 |
| 組織來源 | 腸（空腸） |
| 疾病 | 正常 |
| 傳代比例/細(xì)胞消化 | 1:2傳代 ,，消化1-3分鐘 |
| 培養(yǎng)基配置 | DMEM培養(yǎng)基；10%胎牛血清,；1%雙抗 |
| 簡(jiǎn)介 | 該細(xì)胞從一只新生的未感染豬空腸分離的正常腸上皮細(xì)胞中建立 ,，經(jīng)歷一個(gè)自發(fā)的分化過程 ，在1-2周內(nèi)形成具有低或 高跨上皮電阻（TEER）的極化單層 ,，這取決于加入培養(yǎng)基的血清類型,。 |
| 形態(tài) | 上皮細(xì)胞樣 |
| 生長(zhǎng)特征 | 貼壁生長(zhǎng) |
| 倍增時(shí)間 | 每周 2 至 3 次 |
| 培養(yǎng)條件 | 氣相：空氣 ，95% ,；二氧化碳 ,，5%。 溫度：37攝氏度 ,，培養(yǎng)箱濕度為70%-80%,。 |
| 凍存條件 | 凍存液：90%FBS ，DMSO 10%, 或使用非程序凍存液：貨號(hào)JY-H040 |
| 保藏機(jī)構(gòu) | DSMZ; ACC-701 |
| 產(chǎn)品使用 | 于科學(xué)研究 ,，不可作為動(dòng)物或人類疾病的治療產(chǎn)品使用,。 |
The aim of this study is to determine whether the pretreatment CD8+PD-1+ to CD4+PD-1+ (PERLS) ratio is an independent risk prognostic factor of advanced melanoma patients. We retrospectively analyzed the efficacy and flow cytometry data from advanced melanoma patients who received PD-1 inhibitor as monotherapy between January 1, 2018 and January 26, 2022. Fifty-nine patients were enrolled, the PERLS cutoff was 1.125. PERLS did not correlate with clinical characteristics but were significantly associated with baseline CD8+, CD4+, and CD8+PD-1+ T cells. The mean overall survival and the progression-free survival were 45.8 and 17.1?months for the low PERLS group (n?=?39), compared with 29.9 (P?=?0.001) and 9.7 (P?=?0.003) months for the high PERLS group (n?=?20), respectively. Pretreatment PERLS might contribute to selecting patients before receiving anti-PD-1 therapy.
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