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SVG P12人星形膠質(zhì)細(xì)胞,、
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SVG p12 人星形膠質(zhì)細(xì)胞
Catalogue No.: C605
Product Format: a T25 flask
Culture Properties:貼壁
Complete Growth Medium: 89%H-DMEM+10%FBS+1%雙抗
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Application: Cells and cancer research
NOTE: FOR RESEARCH USE ONLY.
Components
Operation steps for cell culture
19156044901 (同微信)
Corresponding author:WANG Zhiren snowy_
【Abstract】 Objective To investigate the expression of fibroblast growth factor 2(FGF2)and transient receptor potential channel 1(TRPC1)inpatients with schizophrenia and their correlation with psychiatric symptoms.
Catalogue No.: C605
Product Format: a T25 flask
Culture Properties:貼壁
Complete Growth Medium: 89%H-DMEM+10%FBS+1%雙抗
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Application: Cells and cancer research
NOTE: FOR RESEARCH USE ONLY.
Components
Item | Specifications |
a T25 flask | 2X106 |
Manual | 1 copy |
Operation steps for cell culture
- 吸走用于培養(yǎng)基,加入3-5mL PBS后輕輕晃動(dòng)培養(yǎng)瓶潤(rùn)洗細(xì)胞,;
- 吸干凈PBS后加入1mL 0.25%-0.53mM EDTA,,輕輕搖晃培養(yǎng)皿使浸沒(méi)細(xì)胞表面,培養(yǎng)瓶放37度培養(yǎng)箱消化,;
(細(xì)胞對(duì)于消化比較敏感,,消化過(guò)度會(huì)嚴(yán)重影響細(xì)胞狀態(tài),導(dǎo)致細(xì)胞傳代死亡漂浮或者傳代后不長(zhǎng),。細(xì)胞消化到細(xì)胞間隙變大但未脫落,,可以輕輕吹下時(shí)即可終止,禁止消化到細(xì)胞漂浮,?;靹蚣?xì)胞時(shí)盡量輕柔,不要吹出大量氣泡,。) - 加入6-8ml培養(yǎng)基終止消化,,輕輕吹下細(xì)胞混勻;
- 將混勻的細(xì)胞1000rpm(約150g)離心3min,,棄上清,,再用新鮮培養(yǎng)基重懸37度培養(yǎng)箱繼續(xù)培養(yǎng);
![](https://img57.chem17.com/5eceadd4559dcfd25ec7e25d203ec8c39a843434e02bf78495a85229d48eb5f783744a2951dc56a2.png)
19156044901 (同微信)
Corresponding author:WANG Zhiren snowy_
【Abstract】 Objective To investigate the expression of fibroblast growth factor 2(FGF2)and transient receptor potential channel 1(TRPC1)inpatients with schizophrenia and their correlation with psychiatric symptoms.