D283 Med人腦髓母細(xì)胞瘤
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人腦髓母細(xì)胞瘤細(xì)胞D283 Med
種屬 | 人 | ||
別稱 | D283 D283;Med | MED;D283-MED;D283_Med;D-283 283;H283 | Med;D-283MED;D283MED;D283Med;D283-Med;D-283; |
組織來源 | 腦組織 | ||
疾病 | 髓母細(xì)胞瘤 | ||
傳代比例/細(xì)胞消化 | 1:2傳代,懸浮部分離心收集(1000RPM,5分鐘),貼壁部分消化1-2分鐘 | ||
培養(yǎng)基配置 | MEM培養(yǎng)基,;10%胎牛血清,;1%雙抗 | ||
簡(jiǎn)介 | 該細(xì)胞1985年由Friedman等建系,源自一名6歲患有神經(jīng)管細(xì)胞瘤有腹腔轉(zhuǎn)移的白人男性小孩的腹水細(xì)胞 | ||
形態(tài) | 上皮細(xì)胞樣 | ||
生長(zhǎng)特征 | 懸浮聚團(tuán)生長(zhǎng),,有少數(shù)貼壁細(xì)胞 | ||
倍增時(shí)間 | ~52h | ||
基因表達(dá) | The cells produce tumors in nude mice and the resulting tumors are glutamine synthetase positive; neuron specific enolase positive;glial fibrillary acidic proteins negative;S100 (S-100)protein negative;The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase;the brain isoenzyme of creatine kinase;L-DOPA decarboxylase;bombesin-like immunoreactivity) | ||
致瘤性 | Yes,in nude mice(The cells form serially transplantable intercranial and subcutaneous tumors.)Yes,in soft agar | ||
STR | Amelogenin:X,Y CSF1PO:9,12 D13S317:8,10 D16S539:11 D5S818:11 D7S820:10 THO1:7 TPOX:8,11 vWA:16,18 | ||
培養(yǎng)條件 | 氣相:空氣,,95%;二氧化碳,5%,。溫度:37攝氏度,,培養(yǎng)箱濕度為70%-80%。 | ||
凍存條件 | 凍存液:90%FBS,DMSO 10%, 或使用非程序凍存液:貨號(hào)JY-H040 | ||
保藏機(jī)構(gòu) | ATCC;HTB-185 | ||
備注 | 該細(xì)胞為半懸浮和半貼壁細(xì)胞,,懸浮細(xì)胞離心收集,貼壁細(xì)胞消化處理 | ||
產(chǎn)品使用 | 于科學(xué)研究,,不可作為動(dòng)物或人類疾病的治療產(chǎn)品使用,。 |
Both resistance training (RT) and long-duration, high-intensity stretching induce muscular adaptations; however, it is unknown whether the modalities are complementary or redundant, particularly in well-trained individuals. A case-study was conducted on a competitive bodybuilder implementing long-duration, high-intensity stretching of the plantar flexors (60?min 6x/week for 12?weeks) in conjunction with their habitual RT. Ultrasound muscle architecture (muscle thickness [MT], fascicle length [FL], and pennation angle [PA]) measurements were collected at multiple sites at four weekly baseline sessions, six (mid) and 12 (post1) weeks following the commencement of the intervention, and a week after the intervention (post2) while isometric strength and range of motion (RoM) were obtained once at baseline, mid, post1, and post2. 2SD band plots were constructed to determine meaningful changes in MT, FL, and PA from the four baseline measures while percentage and absolute change across each timepoint were calculated for all variables. From baseline to post 1, RoM, strength, and MT increased 25.9%, 11.4%, and 7.4%-23.4%, respectively, while four MT and two PA sites exceeded the threshold for meaningful change. The combined stretching and RT protocols resulted in flexibility, strength, and MT adaptations; however, findings should be generalized with caution given the case-study nature of our investigation.
enriched in soils with high C availatility (tai et al., 2020; Fierer et al., 2007). The increase in the relative atundance of Proteotacteria and tacteroidetes is teneficial for the igprovegent of soil fertility. A large agount of N