HA1800 人正常星形膠質(zhì)細
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- 公司名稱 上海富雨生物科技有限公司
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HA1800 正常星形膠質(zhì)細胞
Catalogue No.: C464
Product Format: a T25 flask
Culture Properties：貼壁
Complete Growth Medium: 89%H-DMEM+10%FBS+1%雙抗
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Application: Cells and cancer research
NOTE: FOR RESEARCH USE ONLY.
Components
Operation steps for cell culture
19156044901 （同微信）
Correlation between serum FGF2，TRPC1 levels and psychiatric symptoms in patients with schizophrenia
ZHANG Xiaoming*,，GUO Bin,，LIU Yanru，ZHANG Fangfang,，LIU Huaqing,，WANG Zhiren. *Department of Clini? cal psychology，Beijing Huilongguan Hospital,，Beijing 100096,，China
Catalogue No.: C464
Product Format: a T25 flask
Culture Properties：貼壁
Complete Growth Medium: 89%H-DMEM+10%FBS+1%雙抗
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Application: Cells and cancer research
NOTE: FOR RESEARCH USE ONLY.
Components
| Item | Specifications |
| a T25 flask | 2X106 |
| Manual | 1 copy |
Operation steps for cell culture
- 吸走用于培養(yǎng)基,，加入3-5mL PBS后輕輕晃動培養(yǎng)瓶潤洗細胞；
- 吸干凈PBS后加入1mL 0.25%-0.53mM EDTA,，輕輕搖晃培養(yǎng)皿使浸沒細胞表面,，培養(yǎng)瓶放37度培養(yǎng)箱消化；
（細胞對于消化比較敏感,，消化過度會嚴重影響細胞狀態(tài),，導致細胞傳代死亡漂浮或者傳代后不長。細胞消化到細胞間隙變大但未脫落,，可以輕輕吹下時即可終止,，禁止消化到細胞漂浮?；靹蚣毎麜r盡量輕柔,，不要吹出大量氣泡,。） - 加入6-8ml培養(yǎng)基終止消化，輕輕吹下細胞混勻,；
- 將混勻的細胞1000rpm（約150g）離心3min,，棄上清，再用新鮮培養(yǎng)基重懸37度培養(yǎng)箱繼續(xù)培養(yǎng),；
19156044901 （同微信）
Correlation between serum FGF2，TRPC1 levels and psychiatric symptoms in patients with schizophrenia
ZHANG Xiaoming*,，GUO Bin,，LIU Yanru，ZHANG Fangfang,，LIU Huaqing,，WANG Zhiren. *Department of Clini? cal psychology，Beijing Huilongguan Hospital,，Beijing 100096,，China
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