Recombinant Epithelial Cell Transforming Sequence 2 (ECT2) 

上皮細(xì)胞轉(zhuǎn)化序列2(ECT2)重組蛋白

[ PROPERTIES ]

Residues: Leu166~Arg348,with N-terminal His-Tag.

Host:E. coli

Subcellular Location: Nucleus. Cytoplasm.cytoskeleton, spindle. Cleavage furrow. Midbody.Cell junction, tight junction.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation:Supplied as lyophilized form in PBS,pH7.4, containing 1mM DTT, 5% trehalose, 0.01%sarcosyl and preservative.

Predicted isoelectric point: 6.4

Predicted Molecular Mass: 22.8kDa

Applications:SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGSEF-LVHHM GGVIRKDFNS KVTHLVANCT QGEKFRVAVS LGTPIMKPEWIYKAWERRNE QDFYAAVDDF RNEFKVPPFQ DCILSFLGFS DEEKTNMEEMTEMQGGKYLPLGDERCTHLV VEENIVKDLP FEPSKKLYVV KQEWFWGSIQ MDARAGETMY LYEKANTPELKKSVSMLSLN TPNSNRKR

[  IDENTIFICATION  ]

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