This antibody will not cross-react with porcine, bovine or sheep.
靶標(biāo)信息
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
應(yīng)用
建議稀釋比
流式細(xì)胞分析 (Flow)
1-4 µg/mL
免疫沉淀 (IP)
Assay-dependent
SSEA1 Antibody (MA1-19094) in Flow
Separation of human neutrophil granulocytes (red-filled) from CD15 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD15 (MEM-158) purified Monoclonal antibody (Product # MA1-19094) (concentration in sample 0.3 µg/mL) GAM APC.