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化工儀器網(wǎng)>產(chǎn)品展廳>實(shí)驗(yàn)室常用設(shè)備>實(shí)驗(yàn)室家具>試劑/樣品/器皿柜> Image-iT™ Green Hypoxia Reage...

Image-iT™ Green Hypoxia Reagent 缺氧監(jiān)測(cè)試劑

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       賽默飛世爾科技賽默飛世爾科技(紐約證交所代碼:TMO)是科學(xué)服務(wù)領(lǐng)域的世界領(lǐng)導(dǎo)者,。公司年銷售額超過(guò)250億美元,。在全球擁有約70,000名員工。我們的使命是攜手客戶,,讓世界更健康,、更清潔、更安全,。我們幫助客戶加速生命科學(xué)領(lǐng)域的研究,、解決在分析領(lǐng)域所遇到的復(fù)雜問(wèn)題與挑戰(zhàn),促進(jìn)醫(yī)療診斷發(fā)展,、加速藥物上市進(jìn)程,、提高實(shí)驗(yàn)室生產(chǎn)力。我們?nèi)虺^(guò)75,000名賽默飛員工將借助于一系列行業(yè)領(lǐng)先的品牌Thermo Scientific,、Applied Biosystems、Invitrogen、Fisher Scientific,、Unity Lab Services和Patheon,,為客戶提供另想的創(chuàng)新技術(shù)、便捷采購(gòu)方案和全方位服務(wù),。

      欲了解更多信息,,請(qǐng)瀏覽公司網(wǎng)站:www.thermofisher.com 賽默飛世爾科技中國(guó)簡(jiǎn)介賽默飛世爾科技進(jìn)入中國(guó)發(fā)展已超過(guò)35年,在中國(guó)的總部設(shè)于上海,,并在北京,、廣州、香港,、成都,、沈陽(yáng)、西安,、南京,、武漢、昆明等地設(shè)立了分公司,,員工人數(shù)約為5000名,。我們的產(chǎn)品主要包括分析儀器、實(shí)驗(yàn)室設(shè)備,、試劑,、耗材和軟件等,提供實(shí)驗(yàn)室綜合解決方案,,為各行各業(yè)的客戶服務(wù),。為了滿足中國(guó)市場(chǎng)的需求,現(xiàn)有7家工廠分別在上海、北京,、蘇州和廣州等地運(yùn)營(yíng),。我們?cè)谌珖?guó)還設(shè)立了8個(gè)應(yīng)用開(kāi)發(fā)中心以及示范實(shí)驗(yàn)室,將世界級(jí)的前沿技術(shù)和產(chǎn)品帶給中國(guó)客戶,,并提供應(yīng)用開(kāi)發(fā)與培訓(xùn)等多項(xiàng)服務(wù),;擁位于上海的中國(guó)創(chuàng)新中心,擁有有100多位專業(yè)研究人員和工程師及70多項(xiàng)專利,。創(chuàng)新中心專注于針對(duì)垂直市場(chǎng)的產(chǎn)品研究和開(kāi)發(fā),,結(jié)合中國(guó)市場(chǎng)的需求和國(guó)外先進(jìn)技術(shù),研發(fā)適合中國(guó)的技術(shù)和產(chǎn)品,;我們擁有遍布全國(guó)的維修服務(wù)網(wǎng)點(diǎn)和特別成立的中國(guó)技術(shù)培訓(xùn)團(tuán)隊(duì),,在全國(guó)有超過(guò)2600名專業(yè)人員直接為客戶提供服務(wù)。我們致力于幫助客戶使世界更健康,、更清潔,、更安全。

      欲了解更多信息,,請(qǐng)登錄網(wǎng)站:www.thermofisher.com 全國(guó)服務(wù)熱線:021-61621892公司電子郵箱:[email protected]

科學(xué)儀器

    描述:

    Image-iT Hypoxia Reagent是一種新型的,、可固定的熒光標(biāo)記物,專用于測(cè)定活細(xì)胞中的缺氧狀態(tài),。當(dāng)活細(xì)胞處于正常氧濃度的環(huán)境中時(shí),,它是不發(fā)熒光的; 當(dāng)氧濃度降低時(shí),它的熒光亮度會(huì)變亮,,可用于實(shí)時(shí)動(dòng)態(tài)監(jiān)測(cè)低氧下的細(xì)胞狀態(tài),。
    可提供兩款缺氧檢測(cè)試劑 Image-iT™ Green Hypoxia Reagent (I14833, I14834) 和 Image-iT™ Red Hypoxia Reagent (H10498):

    • 可動(dòng)態(tài)監(jiān)測(cè):低氧環(huán)境下的活細(xì)胞
    • 兩種顏色可選 Image-iT™ Red Hypoxia 和 Image-iT™ Green Hypoxia,;
    • 可固定:Image-iT Green Hypoxia 可固定但不可逆,而Image-iT™ Red Hypoxia可進(jìn)行缺氧可逆檢測(cè),;
    • 簡(jiǎn)便易用:直接加入培養(yǎng)細(xì)胞中即可成像;
    Analyzing HeLa cells under hypoxic condition CellLight Histone 2B-RFP
    HeLa cells were plated on MatTek dishes at a density of 100k/dish and incubated with CellLight Histone 2B-RFP (red) overnight at 37°C in CO2 incubator. Medium was replaced with fresh growth medium containing Image-iT Green Hypoxia Reagent at a final concentration of 5 µM. Cells were incubated at 20% or 1% oxygen for 3 hrs. The cells were then washed 2X with Live Cell Imaging Solution (LCIS) and incubated with and NucBlue Live nuclear stain (blue). Cells were imaged on an EVOS FL Auto Cell Imaging System.




    Image-iT Green Hypoxia Reagent is a novel, fixable fluorogenic compound for measuring hypoxia in live cells. It is non-fluorescent when live cells are in an environment with normal oxygen concentrations and becomes fluorescent when oxygen levels are decreased. Image-iT Green Hypoxia Reagent sustains its fluorescence when cells/tissue return to normal oxygen levels, allowing the cells/tissue to be fixed with minimal loss of fluorescent signal.

    Features of Image-iT Green Hypoxia Reagent include:
    ? Indicates hypoxia in live cells by fluorescing in low oxygen environments
    ? Fixable—sustains fluorescent signal when cells/tissue return to normal oxygen levels
    ? Easy to use—just add to cell culture media and image

    Image-iT Green Hypoxia Reagent is a fluorogenic compound that is live cell permeable and becomes fluorescent in environments with low oxygen concentrations. These properties makes it a highly useful tool for detecting cells and tissue under hypoxic conditions. Image-iT Green Hypoxia Reagent is a very sensitive oxygen detector. Unlike pimonidazole adducts that respond only to very low oxygen levels, Image-iT Green Hypoxia Reagent begins to fluoresce when atmospheric oxygen levels are less than 5%. It responds quickly to such environments, and the fluorescence is sustained after the oxygen levels return to normal. These properties make Image-iT Green Hypoxia Reagent an ideal tool for detecting hypoxic conditions around tumors, 3D cultures, spheroids, neurons, etc.

    For Research Use Only. Not for use in diagnostic procedures.


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