手持式葉綠素?zé)晒鈨x---FluorPen FP110用于實(shí)驗(yàn)室、溫室和野外快速測量植物葉綠素?zé)晒鈪?shù),，具有便攜性強(qiáng),、精度高、性價(jià)比高等特點(diǎn),；雙鍵操作,，具圖形顯示屏，內(nèi)置鋰電和數(shù)據(jù)存儲(chǔ)，廣泛應(yīng)用于研究植物的光合作用,、脅迫監(jiān)測,、除草劑檢測或突變體篩選，還可用于生物毒理的生物檢測,，如通過不同植物對(duì)土壤或水質(zhì)污染的葉綠素?zé)晒忭憫?yīng),，找出敏感植物作為生物傳感器用于生物檢測。FP110配備多種葉夾型號(hào),，用于不同的樣品與研究,。

應(yīng)用領(lǐng)域

       手持式葉綠素?zé)晒鈨x---FluorPen FP110適用于光合作用研究和教學(xué)，植物及分子生物學(xué)研究,，農(nóng)業(yè),、林業(yè)，生物技術(shù)領(lǐng)域等,。研究內(nèi)容涉及光合活性,、脅迫響應(yīng)、農(nóng)藥藥效測試,、突變篩選等,。

• 植物光合特性研究
• 光合突變體篩選與表型研究
• 生物和非生物脅迫的檢測
• 植物抗脅迫能力或者易感性研究
• 農(nóng)業(yè)和林業(yè)育種、病害檢測,、長勢與產(chǎn)量評(píng)估
• 除草劑檢測
• 教學(xué)

功能特點(diǎn)

• 結(jié)構(gòu)緊湊,、便攜性強(qiáng)，LED光源,、檢測器,、控制單元集成于僅手機(jī)大小的儀器內(nèi)，重量僅188g
• 功能強(qiáng)大,，是葉綠素?zé)晒饧夹g(shù)的高新結(jié)晶產(chǎn)品,，具備了大型熒光儀的所有功能，可以測量所有葉綠素?zé)晒鈪?shù)
• 內(nèi)置了所有通用葉綠素?zé)晒夥治鰧?shí)驗(yàn)程序,，包括3套熒光淬滅分析程序,、3套光響應(yīng)曲線程序、OJIP快速熒光動(dòng)力學(xué)曲線等
• 高時(shí)間分辨率,，可達(dá)10萬次每秒,，自動(dòng)繪出OJIP曲線并給出26個(gè)OJIP–test參數(shù)
• FluorPen專業(yè)軟件功能強(qiáng)大，可下載,、展示葉綠素?zé)晒鈪?shù)圖表,，也可以通過軟件直接控制儀器進(jìn)行測量
• 具備無人值守自動(dòng)監(jiān)測功能
• 內(nèi)置藍(lán)牙與USB雙通訊模塊，GPS模塊,，輸出帶時(shí)間戳和地理位置的葉綠素?zé)晒鈪?shù)圖表
• 配備多種葉夾型號(hào)：固定葉夾式（適于實(shí)驗(yàn)室內(nèi)暗適應(yīng)或夜間快速測量）、分離葉夾式（適用于野外暗適應(yīng)測量）、探頭式（透明光纖探頭,，具備葉片固定裝置,，用于非接觸性測量監(jiān)測或光適應(yīng)條件下的葉綠素?zé)晒獗O(jiān)測）、用戶定制式等
• 可選配野外自動(dòng)監(jiān)測式熒光儀,，防水防塵設(shè)計(jì)

測量程序與功能

• Ft：瞬時(shí)葉綠素?zé)晒?暗適應(yīng)完成后Ft＝F₀
• QY：量子產(chǎn)額,，表示光系統(tǒng)II 的效率，等于Fv/Fm(暗適應(yīng)狀態(tài))或ΦPSII (光適應(yīng)狀態(tài)),。
• OJIP：快速熒光動(dòng)力學(xué)曲線,，用于研究植物暗適應(yīng)后的快速熒光動(dòng)態(tài)變化
• NPQ：熒光淬滅動(dòng)力學(xué)曲線，用于研究植物從暗適應(yīng)到光適應(yīng)狀態(tài)的熒光淬滅變化過程,。
• LC：光響應(yīng)曲線,，用于研究植物對(duì)不同光強(qiáng)的熒光淬滅反應(yīng)。
• PAR：光合有效輻射,，測量環(huán)境中植物生長可以利用的400-700nm實(shí)際光強(qiáng)（限PAR型號(hào)）,。

應(yīng)用案例

2017年4月，美國國家航空*（NASA）新一代*植物培養(yǎng)器（Advanced Plant Habitat,，APH）搭載聯(lián)盟號(hào)MS-04貨運(yùn)飛船抵達(dá)空間站,。宇航員使用FluorPen手持儀葉綠素?zé)晒鈨x在其中開展植物生理學(xué)及太空食物種植（growth of fresh food in space）的研究。

參考文獻(xiàn)

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附：OJIP參數(shù)及計(jì)算公式

Bckg = background

Fo: = F50µs; fluorescence intensity at 50 µs

Fj: = fluorescence intensity at j-step (at 2 ms)

Fi: = fluorescence intensity at i-step (at 60 ms)

Fm: = maximal fluorescence intensity

Fv: = Fm - Fo (maximal variable fluorescence)

Vj = (Fj - Fo) / (Fm - Fo)

Fm / Fo = Fm / Fo

Fv / Fo = Fv / Fo

Fv / Fm = Fv / Fm

Mo = TRo / RC - ETo / RC

Area = area between fluorescence curve and Fm

Sm = area / Fm - Fo (multiple turn-over)

Ss = the smallest Sm turn-over (single turn-over)

N = Sm . Mo . (I / Vj) turn-over number QA

Phi_Po = (I - Fo) / Fm (or Fv / Fm)

Phi_o = I - Vj

Phi_Eo = (I - Fo / Fm) . Phi_o

Phi_Do = 1 - Phi_Po - (Fo / Fm)

Phi_Pav = Phi_Po - (Sm / tFM); tFM = time to reach Fm (in ms)

ABS / RC = Mo . (I / Vj) . (I / Phi_Po)

TRo / RC = Mo . (I / Vj)

ETo / RC = Mo . (I / Vj) . Phi_o)

DIo / RC = (ABS / RC) - (TRo / RC)