24 roundwell microplates 24個(gè)圓孔微孔板

24 roundwell microplates 24個(gè)圓孔微孔板

24個(gè)圓井微孔板（例如康寧公司生產(chǎn)的）CHO細(xì)胞的標(biāo)準(zhǔn)條件：•0.75-1毫升培養(yǎng)液•在225轉(zhuǎn)/分時(shí)的50毫米震動(dòng)幅度,，或在300轉(zhuǎn)/分時(shí)的25毫米震動(dòng)幅度•三明治蓋CR1524a或CR1324a•夾鉗CR1800（可堆疊三個(gè)板+三明治蓋,，（見下面的照片）進(jìn)一步的細(xì)節(jié)：這種平板格式已經(jīng)被用于CHO細(xì)胞很多年了，從CHO細(xì)胞系沒有現(xiàn)在那么健壯開始,。在倫敦大學(xué)學(xué)院和劍橋醫(yī)學(xué)院（英國）的一個(gè)合作項(xiàng)目中,，從康寧和我們的三明治蓋和夾鉗（Silk等人，2010年,，《生物技術(shù)快報(bào)》,，32:73-78）中驗(yàn)證并優(yōu)化了CHO細(xì)胞的培養(yǎng)，軌道振動(dòng)產(chǎn)生溫和的混合（非常低的剪切應(yīng)力）,，因此即使是脆弱的動(dòng)物細(xì)胞系也可以在這些板中培養(yǎng),。這些板的另一個(gè)優(yōu)點(diǎn)（與上面所述的正方形深孔微滴定板相比）是由兩個(gè)壁而不是一個(gè)壁分開的,。進(jìn)一步降低了交叉污染的可能性。此外,，康寧公司生產(chǎn)的24個(gè)圓孔板在聚苯乙烯與蛋白質(zhì)的結(jié)合特性方面具有不同的質(zhì)量（TC處理,、超低附著,、未處理等）

24 roundwell microplates (e.g. from Corning)Standard conditions for CHO-cells:•0.75-1 ml culture volumes •50 mm shaking amplitude at 225 rpm, or •25 mm shaking amplitude at 300 rpm •sandwich covers CR1524a or CR1324a •clamp CR1800 (three plates + sandwich covers can be stacked, see photo below)Further details:This plate format has been used already for many years for CHO-cells, starting in the time that CHO-cell lines were less robust than that they are at present. In a collaborative project of the UCL in London and Medimmune in Cambridge (UK), the c*tion of CHO-cells was validated and optimized in these plates from Corning and our sandwich covers and clamps (Silk et al, 2010, Biotechnology Letters, 32:73–78).Because of the round well format, orbital shaking gives rise to a gentle mixing (very low shear stress), and so even the most vulnerable animal cell lines can be c*ted in these plates.Another advantage of these plates (in comparison to the square-deepwell microtiter plates as described above) is that the cultures are separated by two walls rather than one, which further reduces the chance of cross-contamination.Furthermore, the 24-roundwell plates from Corning are available in various qualities in terms of binding characteristics of the polystyrene for proteins (TC-treated, ultra-low attachment, untreated, etc.)

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