Glass Bottom Dishes玻璃底皿與培養(yǎng)皿

Glass Bottom Dishes玻璃底皿與培養(yǎng)皿

玻璃底皿與培養(yǎng)皿

采用玻璃底皿,，工作距離短,，熒光、共焦,、高N.A.物鏡

以及圖像分析實(shí)驗(yàn)。

中間有縮小的光學(xué)窗口（小于15或11毫米）的加熱元件將提供更好的

您的樣品溫度穩(wěn)定,，非常適合與玻璃底盤和長(zhǎng)距離工作

目標(biāo),。但是，如果使用浸入式物鏡,，這個(gè)小窗口將限制進(jìn)入

整個(gè)底面的大型浸沒(méi)物鏡,。在這種情況下，加熱元件為35mm

應(yīng)使用間隙/窗TC-E35,。使用物鏡仍能提供溫度穩(wěn)定性

加熱器,。

與玻璃底皿相比，玻璃底皿具有良好的熱接觸性,，因?yàn)椴ＡУ诪閒la,，

標(biāo)準(zhǔn)的塑料培養(yǎng)皿通常在底部邊緣有一個(gè)突出的邊緣。這個(gè)

邊緣把盤子舉過(guò)受熱面,。金屬墊片/墊圈可用于消除這種氣隙,，

然而。這將減少浸入式光學(xué)元件到盤底部的通道,，因此TC-E35x15/11可以

僅用于遠(yuǎn)程目標(biāo),。如果使用浸入式光學(xué)元件和大型物鏡，35毫米加熱

元件TC-E35應(yīng)與目標(biāo)加熱器結(jié)合使用,。請(qǐng)注意,，一些玻璃底

碟子，例如馬特克碟子,，是由標(biāo)準(zhǔn)的康寧（或獵鷹碟子）制成的,。

由于不同品牌的菜有不同的外徑，有時(shí)大于38毫米,，我們做

減少插入件以提供更好的fi和與加熱元件內(nèi)部切口的熱接觸,，

38毫米。我們還制作了一個(gè)50毫米的插件,，將培養(yǎng)箱和更大的加熱元件內(nèi)的培養(yǎng)皿居中,，

里面有50毫米的切口,。

圖示為安裝在磁性顯微鏡適配器內(nèi)的加熱元件TC-E35/15/11

Glass Bottom Dishes vs. Petri Dishes
Glass bottom dishes are used with short working distance, high N.A. objective in fluorescence, confocal

and image analysis experiments.
The heating elements with reduced optical window in the middle (down to 15 or 11mm) will provide better
temperature stability for your sample, and are perfect for use with glass bottom dishes and long-working-distance
objectives. If immersion objectives are used, however, this small window will limit access
of the large immersion objective to the whole bottom surface. In this case a heating element with 35mm
clearance/window TC-E35 should be used. Temperature stability still can be provided by using an objective
heater.
In contrasts to glass bottom dishes, which provide good thermal contact because of fla glass bottom,
standard plastic Petri dishes very often have a protruding rim alone the bottom edges of the dish. This
rim elevates the dish above heating surface. Metal shims/washes can be used to eliminate this air gap,
however. This will reduce access for the immersion optics to the bottom of the dish, so TC-E35x15/11 can
be used with long-distance objectives only. If using immersion optics and large objectives, 35mm heating
element TC-E35 should be used, combined with an objective heater. Please note, that some glass bottom
dishes, Mattek dishes for example, are fabricated from standard Corning (or Falcon dishes).
Since different brands of dishes have different outside diameter, sometimes larger than 38mm, we make
reducing inserts to provide better fi and thermal contact to the inside cutout of the heating elements,
which is 38mm. We also make a 50mm insert to center the dishes inside incubators and larger heating elements,
which have 50mm inside cutout.
Shown here is a heating element TC-E35/15/11 mounted inside a magnetic microscope adapter

 注：楊清辰發(fā)布