巨噬細胞集落刺激因子(MCSF)活性蛋白

HZA090Mu01 100µg Active Colony Stimulating Factor 1, Macrophage (MCSF) Organism Species: Mus musculus (Mouse) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 1th Edition (Apr, 2016) [ PROPERTIES ] Source: Prokaryotic expression. Host: E. coli Residues: Lys33~Ser204 Tags: N-terminal His-tag Purity: >95% Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and Proclin300. Predicted isoelectric point: 巨噬細胞集落刺激因子(MCSF)活性蛋白6.0 Predicted Molecular Mass: 23.6kDa Applications: Cell culture; Activity Assays; In vivo assays. (May be suitable for use in other assays to be determined by the end user.) [ USAGE ] Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. [ STORAGE AND STABILITY ] Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 oC for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition. [ SEQUENCE ] [ ACTIVITY ] Measured in a cell proliferation assay using mouse BMDC (bone marrow derived dendritic cells). The ED50 (median effective dose) for this effect is less than 0.25 ng/ml. In-house data of APA090Mu01 used in cellular experiment: 巨噬細胞集落刺激因子(MCSF)活性蛋白Six-eight weeks old Balb/c mice were used for BMDC. At first, mouse femur and tibia were taken out, and then bone marrow was washed out with serum-free RPMI 1640 medium, followed by centrifugation at 1200RPM for 5min (4 oC). ACK buffer was added to get rid of red blood cells, and then, centrifuged at 1200RPM for 5min (4 oC). Cell pellets were collected and re-suspended, the cells were cultured in DMEM medium supplemented with 10% FBS, or DMEM medium supplemented with 10% FBS and MCSF (APA090Mu01) at 37 oC with 5% CO2 in thermostatic incubator.巨噬細胞集落刺激因子(MCSF)活性蛋白 Three days later, cells were observed by microscope, and the result is shown in Figure 1. Figure 1. Effect of MCSF on BMDC. (A) BMDC cultured in DMEM supplemented with 10%FBS; (B) BMDC cultured in DMEM supplemented with 10%FBS and MCSF. A B [ IDENTIFICATION ] Figure 2. Gene Sequencing (extract) Figure 3. SDS-PAGE, Sample: Active recombinant MCSF, Mouse Figure 4. Western Blot, Sample: Recombinant MCSF, Mouse; Antibody: Rabbit Anti-Mouse MCSF Ab (PAA090Mu01)巨噬細胞集落刺激因子(MCSF)活性蛋白

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HZ196Ca01    半乳糖苷酶β(GLβ)重組蛋白        Recombinant Galactosidase Beta (GLb)    Canis familiaris; Canine 

(Dog)
HZ196Fi01    半乳糖苷酶β(GLβ)重組蛋白        Recombinant Galactosidase Beta (GLb)    Danio rerio (Zebrafish)
HZ196Hu01    半乳糖苷酶β(GLβ)重組蛋白        Recombinant Galactosidase Beta (GLb)    Homo sapiens (Human)
HZ196Mu01    半乳糖苷酶β(GLβ)重組蛋白        Recombinant Galactosidase Beta (GLb)    Mus musculus (Mouse)
HZ196Si01    半乳糖苷酶β(GLβ)重組蛋白        Recombinant Galactosidase Beta (GLb)    Rhesus monkey (Simian)
HZ200Hu01    核糖核酸酶H(RNASEH)重組蛋白        Recombinant Ribonuclease H (RNASEH)    Homo sapiens (Human)
HZ203Bo01    前動力蛋白受體1(PKR1)重組蛋白        Recombinant Prokineticin Receptor 1 (PKR1)    Bos taurus; Bovine 

(Cattle)
HZ203Hu01    前動力蛋白受體1(PKR1)重組蛋白        Recombinant Prokineticin Receptor 1 (PKR1)    Homo sapiens (Human)
HZ203Mu01    前動力蛋白受體1(PKR1)重組蛋白        Recombinant Prokineticin Receptor 1 (PKR1)    Mus musculus (Mouse)
HZ203Ra01    前動力蛋白受體1(PKR1)重組蛋白        Recombinant Prokineticin Receptor 1 (PKR1)    Rattus norvegicus 

(Rat)
HZ204Hu01    生長停滯特異性蛋白6(GAS6)重組蛋白        Recombinant Growth Arrest Specific Protein 6 (GAS6)    Homo 

sapiens (Human)
HZ204Mu01    生長停滯特異性蛋白6(GAS6)重組蛋白        Recombinant Growth Arrest Specific Protein 6 (GAS6)    Mus 

musculus (Mouse)
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HZ207Hu01    谷丙轉(zhuǎn)氨酶(ALT)重組蛋白        Recombinant Alanine Aminotransferase (ALT)    Homo sapiens (Human)
HZ207Mu01    谷丙轉(zhuǎn)氨酶(ALT)重組蛋白        Recombinant Alanine Aminotransferase (ALT)    Mus musculus (Mouse)