日韩av大片在线观看欧美成人不卡|午夜先锋看片|中国女人18毛片水多|免费xx高潮喷水|国产大片美女av|丰满老熟妇好大bbbbbbbbbbb|人妻上司四区|japanese人妻少妇乱中文|少妇做爰喷水高潮受不了|美女人妻被颜射的视频,亚洲国产精品久久艾草一,俄罗斯6一一11萝裸体自慰,午夜三级理论在线观看无码

提供：上海安研生物試劑銷售        ：  ,，

心肌黃酶

其他中文名稱：黃遞酶/硫辛酰胺脫氫酶/二氫硫辛酰胺脫氫酶/二氫硫辛酸脫氫酶

英文名稱：Diaphorase

產(chǎn)地：Sigma

CAS：9001-18-7

來源：from Clostridium kluyveri

分子量：102000-110000Da

活性：50units/mg

活力定義：One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor

性狀：淺棕黃色凍干結(jié)晶或呈現(xiàn)熒光的黃綠色粉末，zui適PH值8.5.N-乙基順丁烯二酰亞胺對(duì)其抑制作用,。

用途：生化研究,。測(cè)定乳酸脫氫酶。除了能催化二氫硫辛酰胺或二氫硫辛酸脫氫,，分別稱為硫辛酰胺或硫辛酸外,，也可催化人工電子受體還原，如其些燃料,，鐵氰酸鹽等,。脫去的兩個(gè)氫能將FAD還原為FADH2，再傳遞給NAD+二形成NADH和H+,，該酶主要用于生化研究,。

保存：-20℃

The name "Diaphorase" has been loosely applied to several enzymes which catalyze the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Many different assay procedures and "units" are used.
Diaphorases which are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase （β-NADH specific） as well as lipoic and lipoamide dehydrogenase activities. It is reported to be a single protein. However, Massey reports that "diaphorase" is probably a denatured lipoamide dehydrogenase. Pre-incubation of the pig heart preparation with Cu²⁺ reduces the lipoamide dehydrogenase activity and proportionay increases the β-NADH diaphorase activity. In our laboratory, we have demonstrated this copper effect to some degree on the pig heart enzyme, but no appreciable effect was observed on the Clostridium kluyveri or torula yeast preparations. The lipoamide dehydrogenase:diaphorase ratio is a measure of the denaturation.

Physical form

Supplied as a lyophilized powder containing trehalose