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提供：上海安研生物試劑銷售網(wǎng)    ： ，

過氧化物酶（辣根）

其他中文名稱：辣根過氧化物酶/過氧化酶

英文名稱：Perxidase（horseradish）/HRP/POD

CAS：9003-99-0

來源：horseradish

活性：標抗級,，RZ＞3,≥300u/mg,；RZ＞3,≥300u/mg；RZ～3,≥250u/mg,；RZ≥150u/mg

活力定義：One pyrogallol unit will from 1.0mg purpurogallin from pyrogallol in 20 sec at PH 6.0 at 20℃

性狀：褐色冷凍干粉,，由無色的酶蛋白和棕色的鐵卟啉結合而成的糖蛋白，HRP由多個同功酶組成,，等電點為PH3-9,，酶催化的zui適PH因供氫體不同而稍有差異，但多在PH5左右,。酶溶于水和58%一下飽和度硫酸銨溶液,。HRP的輔基和酶蛋白zui大吸收光譜分別為403nm和275nm，一般以OD403nm/OD275nm的比值RZ表示酶的純度,。RZ值越小,，非酶蛋白就越多。

用途：生化研究,。為酶標抗體主要的原料之一,，廣泛應用于對第二抗體的酶連反應，是生產(chǎn)制造臨床診斷試劑盒的診斷用酶,；可用該酶配制的血糖,、甘油三酯、膽固醇等生化診斷試劑盒及酶免ELISA測定中的酶標抗體能性,。

保存：-20℃

Application

Horseradish peroxidase （HRP） is isolated from horseradish roots （Amoracia rusticana） and belongs to the ferroprotoporphyrin group of peroxidases.. It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Product P8375, type VI, is an essentially salt free lyophilized powder. It is commonly used to determine amounts of glucose and peroxides in solution. It has been used to study sensory input of cervical spinal cord neurons¹.

Biochem/physiol Actions

When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification. Horseradish peroxidase has been shown to slightly reduce the level of inhibition in a cydAB mutant. Known inhibitors are sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd²⁺, Co²⁺, Cu²⁺, Fe³⁺, Mn²⁺, Ni²⁺, and Pb²⁺ ions.

General description

HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight （~44 kDa） includes the polypeptide chain （33,890 Daltons）, hemin plus Ca²⁺ （~700 Daltons）, and carbohydrate （~9,400 Daltons）. At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Horseradish peroxidase is isolated from horseradish roots （Amoracia rusticana） and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight （~44 kDa） includes the polypeptide chain （33,890 Daltons）, hemin plus Ca²⁺ （~700 Daltons）, and carbohydrate （~9,400 Daltons）. At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.